Streptococcus pneumoniaeDeficient in Pneumolysin or Autolysin Has Reduced Virulence in Meningitis
نویسندگان
چکیده
منابع مشابه
Streptococcus pneumoniae deficient in pneumolysin or autolysin has reduced virulence in meningitis.
BACKGROUND The role played by pneumolysin and autolysin in pneumococcal meningitis is poorly understood. METHODS A rat model was used to investigate the disease, in which surgical implantation of a cisternal catheter allowed bacterial instillation and cerebrospinal fluid (CSF) sampling. RESULTS CSF infection of rats with wild-type pneumococci caused meningitis within 26 h, whereas isogenic ...
متن کاملDifferences in virulence of pneumolysin and autolysin mutants constructed by insertion duplication mutagenesis and in-frame deletion in Streptococcus pneumoniae
BACKGROUND Insertion duplication mutagenesis (IDM) and in-frame deletion (IFD) are common techniques for studying gene function, and have been applied to pneumolysin (ply), a virulence gene in Streptococcus pneumoniae (D39). Discrepancies in virulence between the two techniques were observed in both the previous and present studies. This phenomenon was also observed during mutation analysis of ...
متن کاملDecreased virulence of a pneumolysin-deficient strain of Streptococcus pneumoniae in murine meningitis.
Pneumolysin, neuraminidases A and B, and hyaluronidase are virulence factors of Streptococcus pneumoniae that appear to be involved in the pathogenesis of meningitis. In a murine model of meningitis after intracerebral infection using mutants of S. pneumoniae D39, only mice infected with a pneumolysin-deficient strain were healthier at 32 and 36 h, had lower bacterial titers in blood at 36 h, a...
متن کاملMolecular characterization of equine isolates of Streptococcus pneumoniae: natural disruption of genes encoding the virulence factors pneumolysin and autolysin.
Although often considered a strict human pathogen, Streptococcus pneumoniae has been reported to infect and cause pneumonia in horses, although the pathology appears restricted compared to that of human infections. Here we report on the molecular characterization of a group of S. pneumoniae isolates obtained from horses in England and Ireland. Despite being obtained from geographically distinct...
متن کاملCloning of Minor Autolysin of Streptococcus Pneumoniae
Abstract Background and Objective: Increased antibiotic resistant strains and inadequacy of current vaccines against pneumococcal infections necessitate the study of novel protein antigens. It seems that minor autolysin of Streptococcus pneumoniae may have antigenicity. Thus, we aimed at cloning its gene for the first time. Material and Methods: After DNA extraction of Streptococcus pneumoniae ...
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ژورنال
عنوان ژورنال: The Journal of Infectious Diseases
سال: 2008
ISSN: 0022-1899,1537-6613
DOI: 10.1086/527322